Unpackaging the genetics of mammalian fertility: strategies to identify the "reproductive genome".

Document Type

Article

Publication Date

12-2018

JAX Source

Biol Reprod 2018 Dec; 99(6):1119-1128

Volume

99

Issue

6

First Page

1119

Last Page

1128

ISSN

1529-7268

PMID

29878059

DOI

https://doi.org/10.1093/biolre/ioy133

Abstract

Gene mutations, including different alleles of the same gene, are tremendously useful in deconstructing complex developmental systems, such as reproduction, into component molecular pathways. For this reason, great effort has been devoted in the past three decades to biased (reverse genetic) and unbiased (forward genetic) searches for new genes that impact mammalian reproduction and fertility. These efforts have more recently been complemented with international efforts to systematically mutate all mouse genes and to determine their phenotypes (essentially a hybrid of forward and reverse genetics). Here, we survey the available data on the relative productivity of these approaches in identifying fertility genes, estimate the number of protein-coding genes essential for fertility of males and females, and predict the next major directions in the genetics of reproduction and fertility.

Comments

We thank Drs John Eppig, Paula Cohen, Miguel Brieno-Enriquez and Laura Reinholdt for discussion and thoughtful comments on the manuscript, and Zoe Reifsnyder for help with the figures.

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