Dynamic changes in Sox2 spatio-temporal expression promote the second cell fate decision through

Document Type

Article

Publication Date

3-20-2018

JAX Source

Biochem J 2018 Mar 20;475(6):1075-1089

Volume

475

Issue

6

First Page

1075

Last Page

1089

ISSN

1470-8728

PMID

29487166

DOI

https://doi.org/10.1042/BCJ20170418

Grant

Agency for Science Technology & Research

Abstract

Oct4 and Sox2 regulate the expression of target genes such as Nanog, Fgf4, and Utf1, by binding to their respective regulatory motifs. Their functional cooperation is reflected in their ability to heterodimerize on adjacent cis regulatory motifs, the composite Sox/Oct motif. Given that Oct4 and Sox2 regulate many developmental genes, a quantitative analysis of their synergistic action on different Sox/Oct motifs would yield valuable insights into the mechanisms of early embryonic development. In the present study, we measured binding affinities of Oct4 and Sox2 to different Sox/Oct motifs using fluorescence correlation spectroscopy. We found that the synergistic binding interaction is driven mainly by the level of Sox2 in the case of the Fgf4 Sox/Oct motif. Taking into account Sox2 expression levels fluctuate more than Oct4, our finding provides an explanation on how Sox2 controls the segregation of the epiblast and primitive endoderm populations within the inner cell mass of the developing rodent blastocyst.

Biochem J 2018 Mar 20;475(6):1075-1089.

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