3D genomics across the tree of life reveals condensin II as a determinant of architecture type.
Document Type
Article
Publication Date
5-28-2021
Publication Title
Science
Keywords
JMG, Adenosine Triphosphatases, Algorithms, Animals, Biological Evolution, Cell Nucleolus, Cell Nucleus, Centromere, Chromosomes, Chromosomes, Human, DNA-Binding Proteins, Eukaryota, Genome, Genome, Human, Genomics, Heterochromatin, Humans, Interphase, Mitosis, Models, Biological, Multiprotein Complexes, Telomere
JAX Source
Science 2021 May 28; 372(6545):984-989
Volume
372
Issue
6545
First Page
984
Last Page
989
ISSN
1095-9203
PMID
34045355
DOI
https://doi.org/10.1126/science.abe2218
Abstract
We investigated genome folding across the eukaryotic tree of life. We find two types of three-dimensional (3D) genome architectures at the chromosome scale. Each type appears and disappears repeatedly during eukaryotic evolution. The type of genome architecture that an organism exhibits correlates with the absence of condensin II subunits. Moreover, condensin II depletion converts the architecture of the human genome to a state resembling that seen in organisms such as fungi or mosquitoes. In this state, centromeres cluster together at nucleoli, and heterochromatin domains merge. We propose a physical model in which lengthwise compaction of chromosomes by condensin II during mitosis determines chromosome-scale genome architecture, with effects that are retained during the subsequent interphase. This mechanism likely has been conserved since the last common ancestor of all eukaryotes.
Recommended Citation
Hoencamp C,
Dudchenko O,
Elbatsh A,
Brahmachari S,
Raaijmakers J,
van Schaik T,
Sedeño Cacciatore Á,
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van den Broek B,
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Teunissen H,
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Wang M,
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Behringer R,
Kaur P,
Medema R,
van Steensel B,
de Wit E,
Onuchic J,
Di Pierro M,
Lieberman Aiden E,
Rowland B.
3D genomics across the tree of life reveals condensin II as a determinant of architecture type. Science 2021 May 28; 372(6545):984-989