RUNX1-mediated alphaherpesvirus-host trans-species chromatin interaction promotes viral transcription.
Document Type
Article
Publication Date
6-1-2021
Publication Title
Sci Adv
Keywords
JGM
JAX Source
Sci Adv 2021 Jun 23; 7(26):eabf8962
Volume
7
Issue
26
ISSN
2375-2548
PMID
34162542
DOI
https://doi.org/10.1126/sciadv.abf8962
Abstract
Like most DNA viruses, herpesviruses precisely deliver their genomes into the sophisticatedly organized nuclei of the infected host cells to initiate subsequent transcription and replication. However, it remains elusive how the viral genome specifically interacts with the host genome and hijacks host transcription machinery. Using pseudorabies virus (PRV) as model virus, we performed chromosome conformation capture assays to demonstrate a genome-wide specific trans-species chromatin interaction between the virus and host. Our data show that the PRV genome is delivered by the host DNA binding protein RUNX1 into the open chromatin and active transcription zone. This facilitates virus hijacking host RNAPII to efficiently transcribe viral genes, which is significantly inhibited by either a RUNX1 inhibitor or RNA interference. Together, these findings provide insights into the chromatin interaction between viral and host genomes and identify new areas of research to advance the understanding of herpesvirus genome transcription.
Recommended Citation
Xiao K,
Xiong D,
Chen G,
Yu J,
Li Y,
Chen K,
Zhang L,
Xu Y,
Xu Q,
Huang X,
Gao A,
Cao K,
Yan K,
Dai J,
Hu X,
Ruan Y,
Fu Z,
Li G,
Cao G.
RUNX1-mediated alphaherpesvirus-host trans-species chromatin interaction promotes viral transcription. Sci Adv 2021 Jun 23; 7(26):eabf8962