Document Type

Article

Publication Date

7-1-2021

Publication Title

IET Syst Biol

Keywords

JGM, Biomarkers, Breast Neoplasms, Female, Gene Expression Profiling, Gene Expression Regulation, Gene Regulatory Networks, Humans, RNA, Long Noncoding, RNA, Messenger

JAX Source

IET Syst Biol 2021 Jul; 15(5): 137-147

Volume

15

Issue

5

First Page

137

Last Page

147

ISSN

1751-8857

PMID

33991433

DOI

https://doi.org/10.1049/syb2.12020

Abstract

Breast cancer is among the lethal types of cancer with a high mortality rate, globally. Its high prevalence can be controlled through improved analysis and identification of disease-specific biomarkers. Recently, long non-coding RNAs (lncRNAs) have been reported as key contributors of carcinogenesis and regulate various cellular pathways through post-transcriptional regulatory mechanisms. The specific aim of this study was to identify the novel interactions of aberrantly expressed genetic components in breast cancer by applying integrative analysis of publicly available expression profiles of both lncRNAs and mRNAs. Differential expression patterns were identified by comparing the breast cancer expression profiles of samples with controls. Significant co-expression networks were identified through WGCNA analysis. WGCNA is a systems biology approach used to elucidate the pattern of correlation between genes across microarray samples. It is also used to identify the highly correlated modules. The results obtained from this study revealed significantly differentially expressed and co-expressed lncRNAs and their cis- and trans-regulating mRNA targets which include RP11-108F13.2 targeting TAF5L, RPL23AP2 targeting CYP4F3, CYP4F8 and AL022324.2 targeting LRP5L, AL022324.3, and Z99916.3, respectively. Moreover, pathway analysis revealed the involvement of identified mRNAs and lncRNAs in major cell signalling pathways, and target mRNAs expression is also validated through cohort data. Thus, the identified lncRNAs and their target mRNAs represent novel biomarkers that could serve as potential therapeutics for breast cancer and their roles could also be further validated through wet labs to employ them as potential therapeutic targets in future.

Comments

This is an open access article under the terms of the Creative Commons Attribution License.

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