Flow cytometric identification of T fh 13 cells in mouse and human

Authors

Jennifer S Chen
Jessica D S Grassmann, The Jackson LaboratoryFollow
Uthaman Gowthaman
Sam J Olyha
Tregony Simoneau
M Cecilia Berin
Stephanie C Eisenbarth
Adam Williams, The Jackson LaboratoryFollow

Document Type

Article

Publication Date

2-2021

Keywords

JGM

JAX Source

J Allergy Clin Immunol 2021 Feb; 147(2):470-483

Volume

147

Issue

2

First Page

470

Last Page

483

ISSN

1097-6825

PMID

32709424

DOI

https://doi.org/10.1016/j.jaci.2020.04.063

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T_fh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T_fh13 cells in both mice and humans.

Recommended Citation

Chen J, Grassmann J, Gowthaman U, Olyha S, Simoneau T, Berin M, Eisenbarth S, Williams A. Flow cytometric identification of T fh 13 cells in mouse and human J Allergy Clin Immunol 2021 Feb; 147(2):470-483