Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay.

Authors

Kousuke Mouri, The Jackson LaboratoryFollow
Hannah B Dewey, The Jackson LaboratoryFollow
Rodrigo Castro, The Jackson LaboratoryFollow
Daniel Berenzy, The Jackson LaboratoryFollow
Susan Kales, The Jackson LaboratoryFollow
Ryan Tewhey, The Jackson LaboratoryFollow

Document Type

Article

Publication Date

1-11-2023

Original Citation

Mouri K, Dewey H, Castro R, Berenzy D, Kales S, Tewhey R. Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay. Cell Genom. 2023;3(1):100234.

Keywords

JMG, SS1

JAX Source

Cell Genom. 2023;3(1):100234.

ISSN

2666-979X

PMID

36777181

DOI

https://doi.org/10.1016/j.xgen.2022.100234

Abstract

Transcriptional silencers are under- studied compared with activating elements. By using MPRAduo, Mouri et al. perform a whole-genome functional characterization screen of RE1 silencers and identify REST-binding motif characteristics and cofactor localization required for a functional silencer. They also identify human genetic variants that impact RE1 activity.

Comments

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).