Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System.
Document Type
Article
Publication Date
1-1-2023
Original Citation
Gell J,
Shioda T.
Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System. Methods in molecular biology (Clifton, N.J.). 2023; 2677():259
Keywords
JGM, Animals, Humans, Cell Differentiation, Induced Pluripotent Stem Cells, Germ Cells, Gametogenesis, Mammals
ISSN
1940-6029
PMID
37464247
DOI
https://doi.org/10.1007/978-1-0716-3259-8_15
Abstract
Primordial germ cells (PGCs) are the earliest form of mammalian germ lineage. In humans, PGCs are present during a very early and limited window in development, limiting the ability to study fundamental developmental steps in human reproductive biology. However, recent advancements in generating in-vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the generation of hPGCLCs using the incipient mesoderm-like cell (iMeLC) protocol and the subsequent expansion of hPGCLCs in a long-term culture system.