Document Type

Article

Publication Date

12-1-2023

Keywords

JGM, Humans, Antibodies, Monoclonal, Antigens, Surface, Leukemia, Myeloid, Acute, Receptors, Fc, Receptors, IgG, Ribonucleoproteins, Small Nuclear, Tumor Microenvironment

JAX Source

Nat Cancer. 2023;4(12):1675-92.

ISSN

2662-1347

PMID

37872381

DOI

https://doi.org/10.1038/s43018-023-00656-2

Abstract

Despite recent advances in the treatment of acute myeloid leukemia (AML), there has been limited success in targeting surface antigens in AML, in part due to shared expression across malignant and normal cells. Here, high-density immunophenotyping of AML coupled with proteogenomics identified unique expression of a variety of antigens, including the RNA helicase U5 snRNP200, on the surface of AML cells but not on normal hematopoietic precursors and skewed Fc receptor distribution in the AML immune microenvironment. Cell membrane localization of U5 snRNP200 was linked to surface expression of the Fcγ receptor IIIA (FcγIIIA, also known as CD32A) and correlated with expression of interferon-regulated immune response genes. Anti-U5 snRNP200 antibodies engaging activating Fcγ receptors were efficacious across immunocompetent AML models and were augmented by combination with azacitidine. These data provide a roadmap of AML-associated antigens with Fc receptor distribution in AML and highlight the potential for targeting the AML cell surface using Fc-optimized therapeutics.

Comments

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