Document Type
Article
Publication Date
12-2-2023
Original Citation
Nouri N,
Cao R,
Bunsow E,
Nehar-Belaid D,
Marches R,
Xu Z,
Smith B,
Heinonen S,
Mertz S,
Leber A,
Smits G,
van der Klis F,
Mejías A,
Banchereau J,
Pascual V,
Ramilo O.
Young infants display heterogeneous serological responses and extensive but reversible transcriptional changes following initial immunizations. Nat Commun. 2023;14(1):7976.
Keywords
JGM, Humans, Infant, Leukocytes, Mononuclear, Interferons, Vaccination, Gene Expression Profiling, Inflammation
JAX Source
Nat Commun. 2023;14(1):7976.
ISSN
2041-1723
PMID
38042900
DOI
https://doi.org/10.1038/s41467-023-43758-2
Grant
The study was funded by NIH grants AI131386 to O.R. and J.B. and AI168632 to O.R. and V.P.
Abstract
Infants necessitate vaccinations to prevent life-threatening infections. Our understanding of the infant immune responses to routine vaccines remains limited. We analyzed two cohorts of 2-month-old infants before vaccination, one week, and one-month post-vaccination. We report remarkable hetero- geneity but limited antibody responses to the different antigens. Whole-blood transcriptome analysis in an initial cohort showed marked overexpression of interferon-stimulated genes (ISGs) and to a lesser extent of inflammation- genes at day 7, which normalized one month post-vaccination. Single-cell RNA sequencing in peripheral blood mononuclear cells from a second cohort identified at baseline a predominantly naive immune landscape including ISGhi cells. On day 7, increased expression of interferon-, inflammation-, and cytotoxicity-related genes were observed in most immune cells, that reverted one month post-vaccination, when a CD8+ ISGhi and cytotoxic cluster and B cells expanded. Antibody responses were associated with baseline frequencies of plasma cells, B-cells, and monocytes, and induction of ISGs at day 7.
Comments
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