Document Type

Article

Publication Date

11-1-2023

Keywords

JMG, Silene, Magnoliopsida, Chromosomes, Sex Chromosomes, Genome, Mitochondrial

JAX Source

Genome Biol Evol. 2023;15(11):evad192.

ISSN

1759-6653

PMID

37862134

DOI

https://doi.org/10.1093/gbe/evad192

Grant

This project was supported by the National Science Foundation (MCB-2048407) and the National Institutes of Health (R35 GM148134).

Abstract

The angiosperm genus Silene has been the subject of extensive study in the field of ecology and evolution, but the availability of high-quality reference genome sequences has been limited for this group. Here, we report a chromosome-level assembly for the genome of Silene conica based on Pacific Bioscience HiFi, Hi-C, and Bionano technologies. The assembly produced 10 scaffolds (1 per chromosome) with a total length of 862 Mb and only ∼1% gap content. These results confirm previous observations that S. conica and its relatives have a reduced base chromosome number relative to the genus's ancestral state of 12. Silene conica has an exceptionally large mitochondrial genome (>11 Mb), predominantly consisting of sequence of unknown origins. Analysis of shared sequence content suggests that it is unlikely that transfer of nuclear DNA is the primary driver of this mitochondrial genome expansion. More generally, this assembly should provide a valuable resource for future genomic studies in Silene, including comparative analyses with related species that recently evolved sex chromosomes.

Comments

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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