A Unique Role for Protocadherin γC3 in Promoting Dendrite Arborization through an Axin1-Dependent Mechanism.
Document Type
Article
Publication Date
2-8-2023
Original Citation
Steffen D,
Hanes C,
Mah K,
Valiño Ramos P,
Bosch P,
Hinz D,
Radley J,
Burgess RW,
Garrett A,
Weiner J.
A Unique Role for Protocadherin γC3 in Promoting Dendrite Arborization through an Axin1-Dependent Mechanism. J Neurosci. 2023;43(6):918-35.
Keywords
JMG, SS1, Animals, Female, Male, Mice, Axin Protein, Cadherins, Cell Adhesion Molecules, Dendrites, Mice, Knockout, Neuronal Plasticity, Protein Isoforms, Protocadherins
JAX Source
J Neurosci. 2023;43(6):918-35.
ISSN
1529-2401
PMID
36604170
DOI
https://doi.org/10.1523/jneurosci.0729-22.2022
Grant
This work was supported by National Institutes of Health Grants R01 NS055272 (to J.A.W.) and R21 NS090030 (to J.A.W. and R.W.B.).
Abstract
The establishment of a functional cerebral cortex depends on the proper execution of multiple developmental steps, culminating in dendritic and axonal outgrowth and the formation and maturation of synaptic connections. Dysregulation of these processes can result in improper neuronal connectivity, including that associated with various neurodevelopmental disorders. The γ-Protocadherins (γ-Pcdhs), a family of 22 distinct cell adhesion molecules that share a C-terminal cytoplasmic domain, are involved in multiple aspects of neurodevelopment including neuronal survival, dendrite arborization, and synapse development. The extent to which individual γ-Pcdh family members play unique versus common roles remains unclear. We demonstrated previously that the γ-Pcdh-C3 isoform (γC3), via its unique "variable" cytoplasmic domain (VCD), interacts in cultured cells with Axin1, a Wnt-pathway scaffold protein that regulates the differentiation and morphology of neurons. Here, we confirm that γC3 and Axin1 interact in the cortex