Document Type
Article
Publication Date
6-26-2024
Original Citation
Yu CI,
Maser RS,
Marches F,
Banchereau J,
Palucka K.
Protocol to construct humanized mice with adult CD34 STAR Protoc. 2024;5(3):103155.
Keywords
JGM, JMG
JAX Source
STAR Protoc. 2024;5(3):103155.
ISSN
2666-1667
PMID
38935509
DOI
https://doi.org/10.1016/j.xpro.2024.103155
Grant
This work was supported by grants from The Jackson Laboratory Director’s Innovation Fund and NIH (P30 CA034196, R01 CA219880, and R21 OD032454)
Abstract
Humanized mice, defined as mice with human immune systems, have become an emerging model to study human hematopoiesis, infectious disease, and cancer. Here, we describe the techniques to generate humanized NSGF6 mice using adult human CD34 + hematopoietic stem and progenitor cells (HSPCs). We describe steps for constructing and monitoring the engraftment of humanized mice. We then detail procedures for tissue processing and immunophenotyping by flow cytometry to evaluate the multilineage hematopoietic differentiation. For complete details on the use and execution of this protocol, please refer to Yu et al.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.