Document Type

Article

Publication Date

8-20-2024

Keywords

Hepatitis A Virus Cellular Receptor 2, Humans, Animals, CD8-Positive T-Lymphocytes, Drug Resistance, Neoplasm, Mice, Programmed Cell Death 1 Receptor, Prognosis, Biomarkers, Tumor, Immune Checkpoint Inhibitors, Cell Line, Tumor, Female, Male, Lung Neoplasms, Carcinoma, Non-Small-Cell Lung, ADAM10 Protein, Mice, Inbred C57BL, T-Cell Exhaustion

JAX Source

Cell Rep Med. 2024;5(8):101686.

ISSN

2666-3791

PMID

39168104

DOI

https://doi.org/10.1016/j.xcrm.2024.101686

Abstract

Resistance to PD-1 blockade in onco-immunotherapy greatly limits its clinical application. T cell immuno- globulin and mucin domain containing-3 (Tim-3), a promising immune checkpoint target, is cleaved by ADAM10/17 to produce its soluble form (sTim-3) in humans, potentially becoming involved in anti-PD-1 resis- tance. Herein, serum sTim-3 upregulation was observed in non-small cell lung cancer (NSCLC) and various digestive tumors. Notably, serum sTim-3 is further upregulated in non-responding patients undergoing anti- PD-1 therapy for NSCLC and anti-PD-1-resistant cholangiocarcinoma patients. Furthermore, sTim-3 overex- pression facilitates tumor progression and confers anti-PD-1 resistance in multiple tumor mouse models. Mechanistically, sTim-3 induces terminal T cell exhaustion and attenuates CD8 + T cell response to PD-1 blockade through carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1). Moreover, the ADAM10 inhibitor GI254023X, which blocks sTim-3 production, reduces tumor progression in Tim-3 human- ized mice and reverses anti-PD-1 resistance in human tumor-infiltrating lymphocytes (TILs). Overall, human sTim-3 holds great predictive and therapeutic potential in onco-immunotherapy.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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