Adducin Regulates Sarcomere Disassembly During Cardiomyocyte Mitosis.
Document Type
Article
Publication Date
9-3-2024
Original Citation
Xiao F,
Nguyen N,
Wang P,
Li S,
Hsu C,
Thet S,
Kimura W,
Luo X,
Lam N,
Menendez-Montes I,
Elhelaly W,
Cardoso A,
Pereira A,
Singh R,
Sadayappan S,
Kanchwala M,
Xing C,
Ladha F,
Hinson J,
Hajjar R,
Hill J,
Sadek H.
Adducin Regulates Sarcomere Disassembly During Cardiomyocyte Mitosis. Circulation. 2024;150(10):791-805.
Keywords
JGM, Myocytes, Cardiac, Animals, Sarcomeres, Calmodulin-Binding Proteins, Mitosis, Mice, Phosphorylation, Animals, Newborn, Cells, Cultured, Rats, Humans
JAX Source
Circulation. 2024;150(10):791-805.
ISSN
1524-4539
PMID
38708635
DOI
https://doi.org/10.1161/CIRCULATIONAHA.122.059102
Abstract
BACKGROUND: Recent interest in understanding cardiomyocyte cell cycle has been driven by potential therapeutic applications in cardiomyopathy. However, despite recent advances, cardiomyocyte mitosis remains a poorly understood process. For example, it is unclear how sarcomeres are disassembled during mitosis to allow the abscission of daughter cardiomyocytes.
METHODS: Here, we use a proteomics screen to identify adducin, an actin capping protein previously not studied in cardiomyocytes, as a regulator of sarcomere disassembly. We generated many adeno-associated viruses and cardiomyocyte-specific genetic gain-of-function models to examine the role of adducin in neonatal and adult cardiomyocytes in vitro and in vivo.
RESULTS: We identify adducin as a regulator of sarcomere disassembly during mammalian cardiomyocyte mitosis. α/γ-adducins are selectively expressed in neonatal mitotic cardiomyocytes, and their levels decline precipitously thereafter. Cardiomyocyte-specific overexpression of various splice isoforms and phospho-isoforms of α-adducin in vitro and in vivo identified Thr445/Thr480 phosphorylation of a short isoform of α-adducin as a potent inducer of neonatal cardiomyocyte sarcomere disassembly. Concomitant overexpression of this α-adducin variant along with γ-adducin resulted in stabilization of the adducin complex and persistent sarcomere disassembly in adult mice, which is mediated by interaction with α-actinin.
CONCLUSIONS: These results highlight an important mechanism for coordinating cytoskeletal morphological changes during cardiomyocyte mitosis.