Document Type

Article

Publication Date

9-12-2024

Keywords

JMG, Animals, Mice, Hematopoietic Stem Cells, Signal Transduction, Oncostatin M, Oncostatin M Receptor beta Subunit, Sequence Analysis, RNA, Receptors, Oncostatin M, RNA-Seq

JAX Source

Sci Data. 2024;11(1):996.

ISSN

2052-4463

PMID

39266541

DOI

https://doi.org/10.1038/s41597-024-03839-3

Grant

This work was supported by National Institutes of Health grants R01DK118072, R01AG069010, U01AG077925, and a Discovery Research Grant from the Edward P. Evans Foundation to J.J.T. This work was supported in part by the NIH/NCI Cancer Center Support Grant P30CA034196. J.J.T. was supported by a Leukemia & Lymphoma Society Scholar Award and The Dattels Family Endowed Chair. L.S.S. was supported by F31DK127573 and The Tufts University Scheer-Tomasso Fund philanthropic gift.

Abstract

Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmrfl/fl mouse model B6;129-Osmrtm1.1Nat/J. We crossed Osmrfl/fl mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ -dependent and -independent OSM signaling as well as the transcriptional effects of an IL-6 family cytokine on mouse HSCs to further define its anti-inflammatory versus pro-inflammatory properties.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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