Document Type
Article
Publication Date
1-1-2024
Original Citation
Brummaier T,
Rinchai D,
Toufiq M,
Karim M,
Habib T,
Utzinger J,
Paris D,
McGready R,
Marr A,
Kino T,
Terranegra A,
Al Khodor S,
Chaussabel D,
Syed Ahamed Kabeer B.
Design of a targeted blood transcriptional panel for monitoring immunological changes accompanying pregnancy. Front Immunol. 2024;15:1319949.
Keywords
JMG, Pregnancy, Humans, Female, Transcriptome, Postpartum Period, Pregnancy Outcome, RNA, Messenger, Pregnancy Complications
JAX Source
Front Immunol. 2024;15:1319949.
ISSN
1664-3224
PMID
38352867
DOI
https://doi.org/10.3389/fimmu.2024.1319949
Abstract
BACKGROUND: Immunomodulatory processes exert steering functions throughout pregnancy. Detecting diversions from this physiologic immune clock may help identify pregnant women at risk for pregnancy-associated complications. We present results from a data-driven selection process to develop a targeted panel of mRNAs that may prove effective in detecting pregnancies diverting from the norm.
METHODS: Based on a
RESULTS: We identified 176 transcripts that were complemented with eight housekeeping genes. Changes in transcript abundance were seen in the early stages of pregnancy and similar patterns were observed in both datasets. Functional gene annotation suggested significant changes in the lymphoid, prostaglandin and inflammation-associated compartments, when compared to the postpartum controls.
CONCLUSION: The gene panel presented here holds promise for the development of predictive, targeted, transcriptional profiling assays. Such assays might become useful for monitoring of pregnant women, specifically to detect potential adverse events early. Prospective validation of this targeted assay, in-depth investigation of functional annotations of differentially expressed genes, and assessment of common pregnancy-associated complications with the aim to identify these early in pregnancy to improve pregnancy outcomes are the next steps.
Comments
© 2024 Brummaier, Rinchai, Toufiq, Karim, Habib, Utzinger, Paris, McGready, Marr, Kino, Terranegra, Al Khodor, Chaussabel and Syed Ahamed Kabeer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.