In vivo activity of the second-generation proteasome inhibitor ixazomib against pediatric T-cell acute lymphoblastic leukemia xenografts.

Document Type

Article

Publication Date

4-1-2024

Keywords

JCA, JMG, Humans, Child, Animals, Mice, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma, Heterografts, Proteasome Inhibitors, Mice, Inbred NOD, T-Lymphocytes, Mice, SCID, Boron Compounds, Glycine

JAX Source

Exp Hematol. 2024;132:104176.

ISSN

1873-2399

PMID

38320689

DOI

https://doi.org/10.1016/j.exphem.2024.104176

Abstract

The overall survival rate of patients with T-cell acute lymphoblastic leukemia (T-ALL) is now 90%, although patients with relapsed T-ALL face poor prognosis. The ubiquitin−proteasome system maintains normal pro- tein homeostasis, and aberrations in this pathway are associated with T-ALL. Here we demonstrate the in vitro and in vivo activity of ixazomib, a second-generation orally available, reversible, and selective protea- some inhibitor against pediatric T-ALL cell lines and patient-derived xenografts (PDXs) grown orthotopically in immunodeficient NOD.Cg-Prkdc scid IL2rg tm1Wjl/SzJAusb (NSG) mice. Ixazomib was highly potent in vitro, with half-maximal inhibitory concentration (IC50 ) values in the low nanomolar range. As a monotherapy, ixa- zomib significantly extended mouse event-free survival of five out of eight T-ALL PDXs in vivo. © 2024 ISEH – Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.

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