Document Type

Article

Publication Date

4-23-2024

Keywords

Animals, Mice, GTP-Binding Protein alpha Subunits, Gi-Go, Morphogenesis, Hair Cells, Auditory, Cell Polarity, GTP-Binding Protein alpha Subunit, Gi2

JAX Source

Elife. 2024;12:RP88186.

ISSN

2050-084X

PMID

38651641

DOI

https://doi.org/10.7554/eLife.88186

Grant

National Institute on Deafness and Other Communication Disorders R01 DC015242 Basile Tarchini, National Institute on Deafness and Other Communication Disorders R01 DC018304 Basile Tarchini, Fondation Pour l'Audition FPA RD-2018-3 Amandine Jarysta

Abstract

Inhibitory G alpha (GNAI or Gαi) proteins are critical for the polarized morphogenesis of sensory hair cells and for hearing. The extent and nature of their actual contributions remains unclear, however, as previous studies did not investigate all GNAI proteins and included non-physiological approaches. Pertussis toxin can downregulate functionally redundant GNAI1, GNAI2, GNAI3, and GNAO proteins, but may also induce unrelated defects. Here, we directly and systematically determine the role(s) of each individual GNAI protein in mouse auditory hair cells. GNAI2 and GNAI3 are similarly polarized at the hair cell apex with their binding partner G protein signaling modulator 2 (GPSM2), whereas GNAI1 and GNAO are not detected. In

Comments

Copyright Jarysta et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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