Document Type

Article

Publication Date

5-1-2026

Keywords

JMG, SS1, Animals, Mice, Leukemia, Myeloid, Acute, X-Rays, Gamma Rays, Transplantation Chimera, Hematopoietic Stem Cell Transplantation, Mice, Inbred C57BL, Whole-Body Irradiation, Leukocyte Common Antigens

JAX Source

Exp Hematol. 2026;157:105385.

ISSN

1873-2399

PMID

41628795

DOI

https://doi.org/10.1016/j.exphem.2026.105385

Grant

his study was funded in part by National Institute of Health (NIH) grants R01DK118072, R01AG069010, and U01AG077925 to Dr. J. Trowbridgeb. This work was supported in part by the NIH/NCI Cancer Center Support Grant P30CA034196

Abstract

Total body irradiation is routinely used for myeloablation before a mouse hematopoietic cell transplant. Widespread transition from 137Cs g irradiators to x-ray systems has raised questions about whether these modalities yield equivalent biological outcomes. Although prior studies compared g and x-ray irradiation in syngeneic transplants, their performance in reciprocal congenic models and in primary acute myeloid leukemia (AML) transplants remains unclear. Here, we systematically evaluated g and x-ray irradiation across dose conditions and tested dose equivalents in CD45.1/CD45.2 reciprocal transplants and in AML transplant models. Although each modality exhibited distinct early effects, both ultimately supported comparable long-term donor peripheral blood (PB) chimerism in congenic transplants and equivalent AML engraftment, leukemic burden, and disease progression. These findings indicated that, with proper dose calibration, x-ray irradiation is a functionally effective alternative to g irradiation for normal and malignant transplant studies.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

COinS